《植物生理学报》 2013, 49(9): 889-894

二倍体马铃薯耐盐品种鉴定的生理指标测定

张景云^1,3, 缪南生¹, 白雅梅², 赵萍¹, 李文霞³, 吕文河^3,*

¹江西省农业科学院蔬菜花卉研究所, 南昌330200; ²东北农业大学资源与环境学院, 哈尔滨150030; ³东北农业大学农学院, 哈尔滨150030

通信作者：吕文河；E-mail: whlu@neau.edu.cn；Tel: 0451-55191763

摘　要：

以不同耐盐性的二倍体马铃薯富利亚(Solanum phureja, PHU)和窄刀薯(S. stenotomum, STN)杂种(PHU-STN)无性系为材料, 在离体条件下用0 (对照)、10、20、30 mmol·L-1 NaCl进行胁迫处理, 测定试管苗叶的电导率、丙二醛和脯氨酸含量, 结果表明, 随着盐浓度的升高, 这3个生理指标的相对值均逐渐升高, 耐盐性不同的二倍体马铃薯差异极显著。表明电导率、丙二醛和脯氨酸含量可以作为二倍体马铃薯耐盐性鉴定的生理指标。

关键词：二倍体; 马铃薯; 盐胁迫; 生理指标

收稿：2013-04-14   修定：2013-07-04

资助：“十一五”国家“863”项目(2006AA100107)。

Measurement of Physiological Index for Identification of Salt-Tolerant Diploid Potatoes

ZHANG Jing-Yun^1,3, MIAO Nan-Sheng¹, BAI Ya-Mei², ZHAO Ping¹, LI Wen-Xia³, LÜ Wen-He^3,*

¹Institute of Vegetable and Flower, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China; ²College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China; ³College of Agronomy, Northeast Agricultural Uni-versity, Harbin 150030, China

Corresponding author: LÜ Wen-He; E-mail: whlu@neau.edu.cn; Tel: 0451-55191763

Abstract:

Diploid potato Solanum phureja (PHU) and S. stenotomum (STN) hybrids (PHU-STN) with different salt resistances were subject in vitro to 0 (control), 10, 20 and 30 mmol·L^-1 NaCl stress, and electric conductivity, and malondialdehyde (MDA) and proline contents were measured. The results indicated that the three physiological indices increased gradually with an increase in salt concentration, and diploid potatoes with different salt tolerances differed significantly in the three physiological indices. Therefore, electric conductivity, and MDA and proline contents could be used as physiological indices for identification of salt-tolerant diploid clones.

Key words: diploid; potato; salt stress; physiological index